In Florida, purple passion fruit is the most cultivated type, followed by yellow passion fruit to a lesser extent (Bailey et al. incognita was reported to cause damage to both yellow and purple passion fruits in Brazil (Nascimento et al. In Fiji, these RKN species were not able to infect and reproduce on the yellow passionfruit (Kirby 1978). javanica appear to vary in pathogenicity to passion fruit. incognita and the ITS was also 99.7% identical (2 out of 630 bp difference) to OP745246 of M. Based on the BLAST sequence similarity search, the D2-D3 was found 99.7% identical (2 out of 660 bp difference) to MW551794 of M. Two representative sequences, one each for D2-D3 and ITS were submitted to GenBank and received the accession numbers OQ331163 and OQ331162, respectively. 1992) respectively and following the thermal profiles from Singh et al. The D2-D3 and the ITS of rDNA were amplified from the gDNA using the primer pairs D2A: 5´-ACA AGT ACC GTG AGG GAA AGT TG-3´/D3B: 5´-TCC TCG GAA GGA ACC AGC TAC TA-3´ (Nunn 1992), and Vrain2F: 5´-CTT TGT ACA CAC CGC CCG TCG CT-3´/Vrain2R: 5´-TTT CACT CGC CGT TAC TAA GGG AAT C-3´ (Vrain et al. For gDNA extraction from a J2, its cuticle was first punctured using a flamed metallic pin used as a ‘nematode picking tool’ and transferred to 10 μm of 0.05 M NaOH in a PCR tube followed by adding 1 μm of Tween 20 and subjecting the tube at 95 ☌ for 15 min in a thermocycler. The identification was also checked by amplifying the D2-D3 of 28 S and ITS of ribosomal DNA (rDNA) from individual (n = 5) of J2s. A fragment of approximately 290 bp DNA size was amplified only in Mi2F4/Mi1R1 primer, which confirmed the M.

DNA samples were evaluated with a duplex PCR technique using two sets of species-specific primers including SEC-1 F/SEC-1R for M.

Genomic DNA (gDNA) was isolated by smashing individual females (n = 5) in tubes containing PCR-grade water, followed by freezing at -20 ☌ overnight and thawing at 95 ☌ for 1 min.

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